The aforementioned protocols were tested on cats affected by A. abstrusus infection in Italy (Traversa et al., 2009a). A series of studies conducted by Traversa et al. (2018, 2019a, 2019b, 2020a, 2020b) on the efficacy of a combination of imidacloprid 10 % and moxidectin 1 % against A. abstrusus was recently published. The cats were naturally infected; in particular, in eight cats, anthelmintic resistance was evidenced (all the animals received a three-day course of fenbendazole 5 mg/kg orally), and in four cats, a GAP-tested imidacloprid 10 % and moxidectin 1 % spot-on formulation was used for the first time. The formulation was shown to be quite effective in clearing larvae shedding when administered in four doses, starting one day before treatment. After a month of follow-up, positive and negative tests were obtained in 32 and 9 cats, respectively. The treatment eradicated the infection in all the animals with a GAP-tested imidacloprid 10 % and moxidectin 1 % spot-on formulation (Traversa et al., 2018). A similar formulation was subsequently tested in Italy in kittens from three different areas of Abruzzo, Latium and Campania, from April to June. All the cats were treated in four doses, starting one day before treatment. After three months of follow-up, larvae shedding was eradicated in 40 cats, and a positive and a negative test were obtained in nine and 29 cats, respectively (Traversa et al., 2019a). A similar treatment protocol was adopted for cats from Latium, and after a follow-up period of six months, larvae shedding was eradicated in 17 cats, and a positive test was obtained in 1 cat (Traversa et al., 2019b). A similar treatment protocol was adopted for cats from Campania and after a follow-up period of six months, larvae shedding was eradicated in 15 cats, and a positive test was obtained in 2 cats (Traversa et al., 2020a).
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